Dynamic Change in Cells Expressing IL-1β in Rat Hippocampus after Status Epilepticus

نویسندگان

  • Satoru Sakuma
  • Daisuke Tokuhara
  • Hiroshi Otsubo
  • Tsunekazu Yamano
  • Haruo Shintaku
چکیده

BACKGROUND The time course of cytokine dynamics after seizure remains controversial. Here we evaluated the changes in the levels and sites of interleukin (IL)-1β expression over time in the hippocampus after seizure. METHODS Status epilepticus (SE) was induced in adult Wistar rats by means of intraperitoneal injection of kainic acid (KA). Subsequently, the time courses of cellular localization and IL-1β concentration in the hippocampus were evaluated by means of immunohistochemical and quantitative assays. RESULTS On day 1 after SE, CA3 pyramidal cells showed degeneration and increased IL-1β expression. In the chronic phase (>7 days after SE), glial fibrillary acidic protein (GFAP)-positive reactive astrocytes-appeared in CA1 and became IL-1β immunoreactive. Their IL-1β immunoreactivity increased in proportion to the progressive hypertrophy of astrocytes that led to gliosis. Quantitative analysis showed that hippocampal IL-1β concentration progressively increased during the acute and chronic phases. CONCLUSION IL-1β affects the hippocampus after SE. In the acute phase, the main cells expressing IL-1β were CA3 pyramidal cells. In the chronic phase, the main cells expressing IL-1β were reactive astrocytes in CA1.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2014